Part:BBa_K5210015

PMrka

MrkA is a major component of the type III fimbria complex. It is involved in biofilm formation and establishment of infection [1]. Its amino acid sequence is highly conserved among the majority of enterobactereace strains analyzed.Transcription from the mrkA promoter is highly up-regulated by MrkH.PMrkA is an inducer promoter that recognizes and binds to MrkH, and recruits RNA polymerase by transcription factor MrkH to activate the expression of downstream genes[2].

Usage and Biology

In the light Emitting Module, sunlight activation is limited by the UV-B content and it fails to activatie the promoter PumuDC and the downstream LuxAB. So we found an optogenetic tool for far-red light-regulated transcriptional activation apparatus that has been well characterised.

PMrkA is an inducer promoter that recognizes and binds to MrkH, and recruits RNA polymerase by transcription factor MrkH to activate the expression of downstream genes. MRKH recruits RNA polymerase and specifically binds promoter PMrkA, activating transcriptional expression of LuxAB. This part is a part of the composite part BBa_K5210016.

Reference

[1]Wang, Qun et al. “Anti-MrkA Monoclonal Antibodies Reveal Distinct Structural and Antigenic Features of MrkA.” PloS one vol. 12,1 e0170529. 20 Jan. 2017, doi:10.1371/journal.pone.0170529

[2]Yang, Ji et al. “Transcriptional activation of the mrkA promoter of the Klebsiella pneumoniae type 3 fimbrial operon by the c-di-GMP-dependent MrkH protein.” PloS one vol. 8,11 e79038. 14 Nov. 2013, doi:10.1371/journal.pone.0079038

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]